Publications

 
  1. Guan J, Bywaters SM, Brendle SA, Ashley RE, Makhov AM, Conway JF, Christensen ND, Hafenstein S. Cryoelectron Microscopy Maps of Human Papillomavirus 16 Reveal L2 Densities and Heparin Binding Site. Structure. 2017 Feb 7;25(2):253-263. doi: 10.1016/j.str.2016.12.001. PubMed PMID: 28065506.
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  2. Thapa V, Turner GG, Hafenstein S, Overton BE, Vanderwolf KJ, Roossinck MJ. Using a Novel Partitivirus in Pseudogymnoascus destructans to Understand the Epidemiology of White-Nose Syndrome. PLoS Pathog. 2016 Dec 27;12(12):e1006076. doi: 10.1371/journal.ppat.1006076. PubMed PMID: 28027325; PubMed Central PMCID: PMC5189944.
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  3. Carson SD, Hafenstein S, Lee H. MOPS and coxsackievirus B3 stability. Virology. 2017 Jan 15;501:183-187. doi: 10.1016/j.virol.2016.12.002. PubMed PMID: 27940223; PubMed Central PMCID: PMC5218500.
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  4. Organtini LJ, Shingler KL, Ashley RE, Capaldi EA, Durrani K, Dryden KA, Makhov AM, Conway JF, Pizzorno MC, Hafenstein S. Honey bee deformed wing virus structures reveal conformational changes accompany genome release. J Virol. 2016 Nov 16. pii: JVI.01795-16. [Epub ahead of print] PubMed PMID: 27852845.
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  5. Callaway HM, Feng KH, Lee DW, Allison AB, Pinard M, McKenna R, Agbandje-McKenna M, Hafenstein S, Parrish CR. Parvovirus capsid structures required for infection: mutations controlling receptor recognition and protease cleavages. J Virol. 2016 Nov 9. pii: JVI.01871-16. [Epub ahead of print] PubMed PMID: 27847360.
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  6. Hackenbrack N, Rogers MB, Ashley RE, Keel MK, Kubiski SV, Bryan JA, Ghedin E, Holmes EC, Hafenstein SL, Allison AB. Evolution and cryo-EM capsid structure of a North American bat adenovirus and its relationship to other mastadenoviruses. J Virol. 2016 Nov 2. pii: JVI.01504-16. [Epub ahead of print] PubMed PMID: 27807242.
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  7. Hafenstein S. Structural biology: A picornavirus unlike the others. Nat Microbiol. 2016 Oct 26;1(11):16217. doi: 10.1038/nmicrobiol.2016.217. PubMed PMID: 27782143.
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  8. Lee H, Shingler KL, Organtini LJ, Ashley RE, Makhov AM, Conway JF, Hafenstein S. The novel asymmetric entry intermediate of a picornavirus captured with nanodiscs. Sci Adv. 2016 Aug 24;2(8):e1501929. doi: 10.1126/sciadv.1501929. eCollection 2016 Aug. PubMed PMID: 27574701.
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  9. Organtini LJ, Lee H, Iketani S, Huang K, Ashley RE, Makhov AM, Conway JF, Parrish CR, Hafenstein S. The near-atomic resolution structure of a highly neutralizing Fab bound to canine parvovirus. J Virol. 2016 Aug 17. pii: JVI.01112-16. [Epub ahead of print] PubMed PMID: 27535057.
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  10. Hegarty JP, Sangster W, Ashley RE, Myers R, Hafenstein S, Stewart DB Sr. Induction and Purification of C. difficile Phage Tail-Like Particles. Methods Mol Biol. 2016;1476:167-75. doi: 10.1007/978-1-4939-6361-4_12. PubMed PMID: 27507340.
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  11. Liu X, Madhankumar AB, Miller PA, Duck KA, Hafenstein S, Rizk E, Slagle-Webb B, Sheehan JM, Connor JR, Yang QX. MRI contrast agent for targeting glioma: interleukin-13 labeled liposome encapsulating gadolinium-DTPA. Neuro Oncol. 2015 Oct 31. pii: nov263. [Epub ahead of print] PubMed PMID: 26519740.
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  12. Allison AB, Organtini LJ, Zhang S, Hafenstein SL, Holmes EC, Parrish CR. Single mutations in the VP2 300 loop region of the three-fold spike of the carnivore parvovirus capsid can determine host range. J Virol. 2015 Oct 28. pii: JVI.02636-15. [Epub ahead of print] PubMed PMID: 26512077.
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  13. Nishimura Y, McLaughlin NP, Pan J, Goldstein S, Hafenstein S, Shimizu H, Winkler JD, Bergelson JM. The Suramin Derivative NF449 Interacts with the 5-fold Vertex of the Enterovirus A71 Capsid to Prevent Virus Attachment to PSGL-1 and Heparan Sulfate. PLoS Pathog. 2015 Oct 2;11(10):e1005184. doi: 10.1371/journal.ppat.1005184. eCollection 2015 Oct. PubMed PMID: 26430888; PubMed Central PMCID: PMC4592248.
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  14. Guan J, Bywaters SM, Brendle SA, Lee H, Ashley RE, Christensen ND, Hafenstein S. The U4 Antibody Epitope on Human Papillomavirus 16 Identified by Cryo-electron Microscopy. J Virol. 2015 Dec 1;89(23):12108-17. doi: 10.1128/JVI.02020-15. Epub 2015 Sep 23. PubMed PMID: 26401038.
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  15. Guan J, Bywaters SM, Brendle SA, Lee H, Ashley RE, Makhov AM, Conway JF, Christensen ND, Hafenstein S. Structural comparison of four different antibodies interacting with human papillomavirus 16 and mechanisms of neutralization. Virology. 2015 May 18;483:253-263. doi: 10.1016/j.virol.2015.04.016. [Epub ahead of print] PubMed PMID: 25996608.
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  16. Bordería AV, Isakov O, Moratorio G, Henningsson R, Agüera-González S, Organtini L, Gnädig NF, Blanc H, Alcover A, Hafenstein S, Fontes M, Shomron N, Vignuzzi M. Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype. PLoS Pathog. 2015 May 5;11(5):e1004838. doi: 10.1371/journal.ppat.1004838. eCollection 2015 May. PubMed PMID: 25941809; PubMed Central PMCID: PMC4420505.
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  17. Shingler KL, Cifuente JO, Ashley RE, Makhov AM, Conway JF, Hafenstein S. The Enterovirus 71 procapsid binds neutralizing antibodies and rescues virus infection in vitro. J Virol. 2014 Nov 26. pii: JVI.03098-14. [Epub ahead of print] PubMed PMID: 25428877.
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  18. Organtini LJ, Allison AB, Lukk T, Parrish CR, Hafenstein S. Global displacement of canine parvovirus by a host-adapted variant: A structural comparison between pandemic viruses with distinct host ranges. J Virol. 2014 Nov 19. pii: JVI.02611-14. [Epub ahead of print] PubMed PMID: 25410876.
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  19. Lee H, Brendle SA, Bywaters SM, Guan J, Ashley RE, Yoder JD, Makhov AM, Conway JF, Christensen ND, Hafenstein S. A CryoEM study identifies the complete H16.V5 epitope and reveals global conformational changes initiated by binding of the neutralizing antibody fragment. J Virol. 2014 Nov 12. pii: JVI.02898-14. [Epub ahead of print] PubMed PMID: 25392224.
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  20. Pan J, Zhang L, Organtini L, Hafenstein S, Bergelson JM. Specificity of coxsackievirus B3 interaction with human, but not murine, decay accelerating factor (DAF): replacement of a single residue within short consensus repeat 2 prevents virus attachment. J Virol. 2014 Nov 12. pii: JVI.02798-14. [Epub ahead of print] PubMed PMID: 25392210.
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  21. Shingler KL, Organtini LJ, Hafenstein S. Enterovirus 71 Virus Propagation and Purification. Bio Protoc. 2014 May 5;4(9). pii: e1117. PubMed PMID: 27547789.
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  22. Organtini LJ, Makhov AM, Conway JF, Hafenstein S, Carson SD. Kinetic and structural analysis of coxsackievirus b3 receptor interactions and formation of the a-particle. J Virol. 2014 May;88(10):5755-65. doi: 10.1128/JVI.00299-14. Epub 2014 Mar 12. PubMed PMID: 24623425.
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  23. Lee H, Cifuente JO, Ashley RE, Conway JF, Makhov AM, Tano Y, Shimizu H, Nishimura Y, Hafenstein S. A strain-specific epitope of Enterovirus 71 identified by cryoEM of the complex with Fab from neutralizing antibody. J Virol. 2013 Aug 14. [Epub ahead of print] PubMed PMID: 23946455.
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  24. Nishimura Y, Lee H, Hafenstein S, Kataoka C, Wakita T, Bergelson JM, Shimizu H. Enterovirus 71 Binding to PSGL-1 on Leukocytes: VP1-145 Acts as a Molecular Switch to Control Receptor Interaction. PLoS Pathog. 2013 Jul;9(7):e1003511. doi: 10.1371/journal.ppat.1003511. Epub 2013 Jul 25. PubMed PMID: 23935488; PubMed Central PMCID: PMC3723564.
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  25. Gullberg M, Muszynski B, Organtini LJ, Ashley RE, Hafenstein SL, Belsham GJ, Polacek C. Assembly and characterization of foot-and-mouth disease virus empty capsid particles expressed within mammalian cells. J Gen Virol. 2013 Jun 5. [Epub ahead of print] PubMed PMID: 23740480.
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  26. Cifuente JO, Lee H, Yoder JD, Shingler KL, Carnegie MS, Yoder JL, Ashley RE, Makhov AM, Conway JF, Hafenstein S. The EV71 strain 1095 structures of procapsid and mature virion. J. Virol 2013 May 1. [Epub ahread of print] PubMed PMID: 23637404.
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  27. Shingler KL, Yoder JL, Carnegie MS, Ashley RE, Makhov AM, Conway JF, Hafenstein SL. The Enterovirus 71 A-Particle Forms a Gateway to Allow Genome Release: A CryoEM Study of Picornavirus Uncoating. PLoS Pathog. 2013 Mar;9(3):e1003240. doi: 10.1371/journal.ppat.1003240. Epub 2013 Mar 21. PubMed PMID: 23555253; PubMed Central PMCID: PMC3605244.
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  28. Yoder JD, Cifuente JO, Pan J, Bergelson JM, Hafenstein S. The crystal structure of a coxsackievirus B3-RD variant and a refined 9-angstrom cryo-electron microscopy reconstruction of the virus complexed with decay-accelerating factor (DAF) provide a new footprint of DAF on the virus surface. J Virol. 2012 Dec;86(23):12571-81. doi: 10.1128/JVI.01592-12. Epub 2012 Sep 12. PMID: 22973031.
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  29. Stucker KM, Pagan I, Cifuente JO, Kaelber JT, Lillie TD, Hafenstein S, Holmes EC, Parrish CR. The Role of Evolutionary Intermediates in the Host Adaptation of Canine Parvovirus. J Virol. 2011 Nov 23. [Epub ahead of print] PMID: 22114336.
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  30. Ning X, Nguyen D, Mentzer L, Adams C, Lee H, Ashley R, Hafenstein S, Hu J. Secretion of genome-free hepatitis B virus--single strand blocking model for virion morphogenesis of para-retrovirus. PLoS Pathog. 2011 Sep;7(9):e1002255. Epub 2011 Sep 22. PubMed PMID: 21966269; PubMed Central PMCID: PMC3178560.
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  31. Cherwa JE Jr, Organtini LJ, Ashley RE, Hafenstein SL, Fane BA. In Vitro Assembly of the φ174 Procapsid from External Scaffolding Protein Oligomers and Early Pentameric Assembly Intermediates. J Mol Biol. 2011 Aug 5. [Epub ahead of print] PubMed PMID: 21840317.
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  32. Cifuente JO, Ferrer MF, de Giusti CJ, Song WC, Romanowski V, Hafenstein SL, Gómez RM. Molecular determinants of disease in coxsackievirus B1 murine infection. J Med Virol. 2011 Sep;83(9):1571-81. doi: 10.1002/jmv.22133. PubMed PMID: 21739448.
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  33. Pan J, Narayanan B, Shah S, Yoder JD, Cifuente JO, Hafenstein S, Bergelson JM. Single amino acid changes in the virus capsid permit coxsackievirus B3 to bind decay accelerating factor. J Virol. 2011 May 11. [Epub ahead of print] PubMed PMID: 21561916.
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  34. Plevka P, Hafenstein S, Li L, D'Abramo A Jr, Cotmore SF, Rossmann MG, Tattersall P. Structure of a packaging defective mutant of Minute Virus of Mice indicates that the genome is packaged via a pore at a fivefold axis. J Virol. 2011 Mar 2. [Epub ahead of print] PubMed PMID: 21367911.
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  35. Carson SD, Chapman NM, Hafenstein S, Tracy S. Variation of coxsackievirus B3 capsid primary structure, ligands, and stability are selected in a coxsackievirus and adenovirus receptor-limited environment. J Virol. 2011 Jan 26. [Epub ahead of print] PubMed PMID: 21270163.
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  36. Plevka, P.*, Hafenstein, S.*, Harris, K., Cifuente, J.O., Zhang, Y., Bowman, V.D., Chipman, P.R., Bator, C.M., Lin, F., Medof, E., Rossmann., M.G. The interaction of Decay-accelerating factor with Echovirus 7. 2010, J. Virol., in press
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  37. Gullberg, M., Tolf, C., Jonsson, N., Mulders, M.N., Savolainen-Kopra, C., Hovi, T., Van Ranst, M., Lemey, P., Hafenstein, S., and Lindberg, M. Characterization of a putative ancestor of coxsackievirus B5. J Virol. 2010 Jul 14. [Epub ahead of print] PMID: 20631132
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  38. Gullberg, M., Tolf, C., Jonsson, N., Polacek, C., Precechtelova, J., Badurova, M., Sojka, M., Mohlin, C., Israelsson, K., Johannsson, S., Bopegamage, S., Hafenstein, S., and Lindberg, A.M. A single coxsackievirus B2 capsid residue controls cytolysis and apoptosis in rhabdomyosarcoma cells. J Virol. 2010 Jun;84(12):5868-79. Epub 2010 Apr 7, PMID: 20375176
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  39. Goodman, L.B., Lyi, S.M., Johnson, N.C., Cifuente, J.O., Hafenstein, S.L., and Parrish, C.R. The binding site on the transferrin receptor for the parvovirus capsid, and the effects of altered affinity on cell uptake and infection. J Virol. 2010 May;84(10):4969-78. Epub 2010 Mar 3.
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  40. Cotmore, S. F., S Hafenstein, Peter Tattersall. Depletion of virion-associated divalent cations induces Parvovirus Minute Virus of Mice (MVM) to eject its genome in a 3'-to-5' direction from otherwise intact viral particles. 2009 J. Virol. Vol. 84: 1945-1956.
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  41. Hafenstein S, Bowman VD, Sun T, Nelson CD, Palermo LM, Chipman PR, Battisti AJ, Parrish CR, Rossmann MG. Structural comparison of different antibodies interacting with parvovirus capsids. J Virol. 2009 Jun;83(11):5556-66. doi: 10.1128/JVI.02532-08. Epub 2009 Mar 25. PubMed PMID: 19321620; PubMed Central PMCID: PMC2681957.
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  42. Xiao, C., Kuznetsov, Y., Sun, S., Hafenstein, S., Kostyuchenko, V., Chipman, P., Suzan-Monti, M., Raoult, D., McPherson, A., and Rossmann, M. Structural Studies of the Giant Mimivirus. PLoS Biology. Vol. 7, No. 4, e92 doi:10.1371/journal.pbio.1000092
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  43. Zhang, P., Mueller, S., Morais, M. C., Bator, C. M., Bowman, V. D., Hafenstein, S., Wimmer, E., and Rossmann, M. G. Crystal structure of CD155 and electron microscopic studies of its complexes with polioviruses. 2008 PNAS Nov25; 105(47);18284-9.
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  44. Hafenstein, S., Chipman, P. R., Bowman, V. D., Kelly, C. B., Lin, F., Medof, D.E. and Rossmann, M. G. Interaction of decay-accelerating factor with coxsackievirus B3. 2007 J. Virol. Dec: 81 (23): 12927-35.
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  45. Hafenstein, S., Palermo, L. M., Xiao, C., Kostyuchenko, V. A., Morais, M., Nelson, C. D. S., Chipman, P. R., Bowman, V. D., Battisti, A. J., Parrish, C.R., Rossmann, M.G. 2007. Asymmetric binding of transferrin receptor to parvovirus capsids PNAS U S A. 104:6585-9.
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  46. Nelson, C.D., Palermo, L.M., Hafenstein, S.L., Parrish, C.R. 2007. Different mechanisms of antibody-mediated neutralization of parvoviruses revealed using the Fab fragments of monoclonal antibodies. 2007 Virology. 361:283-93.
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  47. Rossmann, M.G., Arisaka, F., Battisti, A.J., Bowman, V.D., Chipman, P.R., Fokine, A., Hafenstein, S., Kanamaru, S., Kostyuchenko, V.A., Mesyanzhinov, V.V., Shneider, M.M., Morais, M.C., Leiman, P.G., Palermo, L.M., Parrish, C.R., Xiao C. 2007. From structure of the complex to understanding of the biology. Acta Crystallogr D Biol Crystallogr. 63: 9-16.
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  48. Palermo, L.M., Hafenstein, S.L., Parrish, C.R. 2006. Purified feline and canine transferrin receptors reveal complex interactions with the capsids of canine and feline parvoviruses that correspond to their host ranges. J Virol. 80: 8482-92.
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  49. Hafenstein, S. L., Chen, M., Fane, B. A. 2004. Genetic and functional analyses of the phiX174 DNA binding protein: the effects of substitutions for amino acid residues that spatially organize the two DNA binding domains. Virol. 318: 204-13.
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  50. Bernal, R. A., Hafenstein, S., Olson, N. H., Bowman, V.D., Chipman, P.R., Baker, T. S., Fane, B. A., Rossmann, M. G. 2003. Structural studies of bacteriophage alpha3 assembly. J. Mol. Biol. 325: 11-24.
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  51. Bernal, R. A., Hafenstein, S., Esmeralda, R., Fane, B. A, Rossmann, M. G. 2003. The phiX174 protein J mediates DNA packaging and viral attachment to host cells. J. Mol. Biol. 337: 1109-1122.
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  52. Brentlinger, K. L., Hafenstein, S., Novak, C. R., Fane, B. A., Borgon, R., McKenna, R., Agbandje-McKenna, M. 2002. Microviridae, a family divided. Isolation, characterization and genome sequence of phiMH2K, a bacteriophage of the obligate intracellular parasitic bacterium Bdellovibrio bacteriovirus. J. Bacteriol. 184: 1089-94.
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  53. Hafenstein, S., Fane, B. A. 2002. phiX174 genome-capsid interactions influence the biophysical properties of the virion: evidence for a scaffolding-like function of the genome during the final stages of morphogenesis. J. Virol. 76: 5350-6.
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